Specific cleavage analysis of mammalian mitochondrial DNA.
نویسندگان
چکیده
منابع مشابه
Cleavage of DNA by Mammalian DNA Topoisomerase
Using the P4 unknotting assay, DNA topoisomerase I1 has been purified from several mammalian cells. Similar to prokaryotic DNA gyrase, mammalian DNA topoisomerase I1 can cleave double-stranded DNA and be trapped as a covalent protein-DNA complex. This cleavage reaction requires protein denaturant treatment of the topoisomerse 11-DNA complex and is reversible with respect to salt and temperature...
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Mitochondrial forms of uracil DNA glycosylase and UV endonuclease have been purified and characterized from the mouse plasmacytoma cell line, MPC-11. As in other cell types, the mitochondrial uracil DNA glycosylase has properties very similar to those of the nuclear enzyme, although in this case the mitochondrial activity was also distinguishable by extreme sensitivity to dilution. Three mitoch...
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Sergentomyia sintoni is the natural vector of Sauroleishmania species of lizards. This sandfly isabundance in and around the burrows of great gerbils. S. sintoni was collected from peridomestic animalshelters, inside and around houses and also from the nearby burrows of the gerbil reservoir hosts,Rhombomys opimus, in several provinces of Iran. Mitochondrial Cytochrome b (Cyt b) of sandflies, wh...
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In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA-Fe(II) [P5E*Fe(lI)] at 0.5 ILM cleaves pBR322 plasmid DNA (50 pAM in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel electrophoresis. High-resolution denaturing gel electrophoresis of a nP-end-labeled 517-base-pair restriction fragment containing a major cleavage site...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1975
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.72.11.4496